Reference - PMID:31147914 - Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation.
Reference summary
- PubMed ID
- PMID:31147914
- Title
- Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation.
- Authors
- Makrantoni V, Robertson D, Marston AL
- Citation
- Methods Mol Biol 2019;2004:119-138
- Publication year
- 2019
- Abstract
- A plethora of biological processes like gene transcription, DNA replication, DNA recombination, and chromosome segregation are mediated through protein-DNA interactions. A powerful method for investigating proteins within a native chromatin environment in the cell is chromatin immunoprecipitation (ChIP). Combined with the recent technological advancement in next generation sequencing, the ChIP assay can map the exact binding sites of a protein of interest across the entire genome. Here we describe a-step-by step protocol for ChIP followed by library preparation for ChIP-seq from yeast cells.
