Reference - PMID:31260531 - Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave.
Reference summary
- PubMed ID
- PMID:31260531
- Title
- Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave.
- Authors
- González-Medina A, Hidalgo E, Ayté J
- Citation
- Nucleic Acids Res 2019 Sep 19;47(16):8439-8451
- Publication year
- 2019
- Abstract
- In fission yeast, MBF-dependent transcription is inactivated at the end of S phase through a negative feedback loop that involves the co-repressors, Yox1 and Nrm1. Although this repression system is well known, the molecular mechanisms involved in MBF activation remain largely unknown. Compacted chromatin constitutes a barrier to activators accessing promoters. Here, we show that chromatin regulation plays a key role in activating MBF-dependent transcription. Gcn5, a part of the SAGA complex, binds to MBF-regulated promoters through the MBF co-activator Rep2 in a cell cycle-dependent manner and in a reverse correlation to the binding of the MBF co-repressors, Nrm1 or Yox1. We propose that the co-repressors function as physical barriers to SAGA recruitment onto MBF promoters. We also show that Gcn5 acetylates specific lysine residues on histone H3 in a cell cycle-regulated manner. Furthermore, either in a gcn5 mutant or in a strain in which histone H3 is kept in an unacetylated form, MBF-dependent transcription is downregulated. In summary, Gcn5 is required for the full activation and correct timing of MBF-regulated gene transcription.
