Reference - PMID:31371524 - Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex.
Reference summary
- PubMed ID
- PMID:31371524
- Title
- Subunit interactions and arrangements in the fission yeast Mis16-Mis18-Mis19 complex.
- Authors
- Korntner-Vetter M, Lefèvre S, Hu XW, George R, Singleton MR
- Citation
- Life Sci Alliance 2019 Aug;2(4)
- Publication year
- 2019
- Abstract
- Centromeric chromatin in fission yeast is distinguished by the presence of nucleosomes containing the histone H3 variant Cnp1 CENP-A Cell cycle-specific deposition of Cnp1 requires the Mis16-Mis18-Mis19 complex, which is thought to direct recruitment of Scm3-chaperoned Cnp1/histone H4 dimers to DNA. Here, we present the structure of the essential Mis18 partner protein Mis19 and describe its interaction with Mis16, revealing a bipartite-binding site. We provide data on the stoichiometry and overall architecture of the complex and provide detailed insights into the Mis18-Mis19 interface.
