Reference - PMID:31477575 - Modulation of TOR complex 2 signaling by the stress-activated MAPK pathway in fission yeast.
Reference summary
- PubMed ID
- PMID:31477575
- Title
- Modulation of TOR complex 2 signaling by the stress-activated MAPK pathway in fission yeast.
- Authors
- Morigasaki S, Chin LC, Hatano T, Emori M, Iwamoto M, Tatebe H, Shiozaki K
- Citation
- J Cell Sci 2019 Oct 10;132(19)
- Publication year
- 2019
- Abstract
- Sin1 is a substrate-binding subunit of target of rapamycin complex 2 (TORC2), an evolutionarily conserved protein kinase complex. In fission yeast, Sin1 has also been identified as a protein that interacts with Spc1 (also known as Sty1) in the stress-activated protein kinase (SAPK) pathway. Therefore, this study examined the relationship between TORC2 and Spc1 signaling. We found that the common docking (CD) domain of Spc1 interacts with a cluster of basic amino acid residues in Sin1. Although diminished TORC2 activity in the absence of the functional Spc1 cascade suggests positive regulation of TORC2 by Spc1, such regulation appears to be independent of the Sin1-Spc1 interaction. Hyperosmotic stress transiently inhibits TORC2, and its swift recovery is dependent on Spc1, the transcription factor Atf1, and the glycelrol-3-phosphate dehydrogenase Gpd1, whose expression is induced upon osmostress by the Spc1-Atf1 pathway. Thus, cellular adaptation to osmostress seems important for TORC2 reactivation, though Spc1 and Atf1 contribute to TORC2 activation also in the absence of osmostress. These results indicate coordinated actions of the SAPK and TORC2 pathways, both of which are essential for fission yeast cells to survive environmental stress.
