Reference - PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Reference summary
- PubMed ID
- PMID:32062975
- Title
- Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
- Authors
- Wu M, Feng G, Zhang B, Xu K, Wang Z, Cheng S, Chang C, Vyas A, Tang Z, Liu X
- Citation
- J Proteome Res 2020 Apr 03;19(4):1776-1787
- Publication year
- 2020
- Abstract
- As the ortholog of human SR protein kinase 1 in fission yeast Schizosaccharomyces pombe , Dsk1 specifically phosphorylates SR proteins (serine/arginine-rich proteins) and promotes splicing of nonconsensus introns. The SRPK (SR protein-specific kinase) family performs highly conserved functions in eukaryotic cells including cell proliferation, differentiation, development, and apoptosis. Although Dsk1 was originally identified as a mitotic regulator, its specific targets involved in cell cycle have yet been unexplored. In this study, using a phosphoproteomics approach, we examined differential protein phosphorylation between wild-type cells and dsk1 -deletion mutants. We found reduced phosphorylation of 149 peptides corresponding to 133 proteins in the dsk1 -null cells. These proteins are involved in various cellular processes, including cytoskeleton organization and signal transduction, and specifically enriched in multiple steps of cell cycle control. Further, targeted MS analyses and in vitro biochemical assays established Cdr2 protein kinase and kinesin motor Klp9 as novel substrates of Dsk1, which function in cell size control for mitotic entry and in chromosome segregation for mitotic exit, respectively. The phosphoprotein networks mediated by Dsk1 reveal, for the first time, the molecular links connecting Dsk1 to mitotic phase transition, sister-chromatid segregation, and cytokinesis, providing further evidence of Dsk1's diverse influence on cell cycle progression and regulation.
