Reference - PMID:3329047 - Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents.

Reference summary

PubMed ID

PMID:3329047

Title

Induction of yeast DNA ligase genes in exponential and stationary phase cultures in response to DNA damaging agents.

Authors

Johnson AL, Barker DG, Johnston LH

Citation

Curr Genet 1986;11(2):107-12

Publication year

1986

Abstract

UV-irradiation of stationary phase cells of Saccharomyces cerevisiae and Schizosaccharomyces pombe leads to a 9-fold and 90-fold increase in transcript levels from the respective DNA ligase genes CDC9 and CDC17, whereas exponential cells show only 3-fold and 2-fold increases. Induction of CDC9 after MMS treatment and gamma-irradiation was also observed by using a CDC9-lacZ translational fusion and assaying for beta-galactosidase. Surprisingly, irradiation of S. cerevisiae induces only a 50% increase in DNA ligase itself, probably reflecting the extremely high in vivo stability of the enzyme. The UV-induction of ligase may be part of a "fail-safe" mechanism which, together with the enzyme stability, ensures adequate supplies of this essential enzyme.

Annotation