Reference - PMID:34208949 - Mapping and Analysis of Swi5 and Sfr1 Phosphorylation Sites.

Reference summary

PubMed ID

PMID:34208949

Title

Mapping and Analysis of Swi5 and Sfr1 Phosphorylation Sites.

Authors

Sevcovicova A, Plava J, Gazdarica M, Szabova E, Huraiova B, Gaplovska-Kysela K, Cipakova I, Cipak L, Gregan J

Citation

Genes (Basel) 2021 Jun 30;12(7)

Publication year

2021

Abstract

The evolutionarily conserved Swi5-Sfr1 complex plays an important role in homologous recombination, a process crucial for the maintenance of genomic integrity. Here, we purified Schizosaccharomyces pombe Swi5-Sfr1 complex from meiotic cells and analyzed it by mass spectrometry. Our analysis revealed new phosphorylation sites on Swi5 and Sfr1. We found that mutations that prevent phosphorylation of Swi5 and Sfr1 do not impair their function but swi5 and sfr1 mutants encoding phosphomimetic aspartate at the identified phosphorylation sites are only partially functional. We concluded that during meiosis, Swi5 associates with Sfr1 and both Swi5 and Sfr1 proteins are phosphorylated. However, the functional relevance of Swi5 and Sfr1 phosphorylation remains to be determined.

Annotation