Reference - PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Reference summary

PubMed ID

PMID:34296454

Title

The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Authors

Mak T, Jones AW, Nurse P

Citation

EMBO J 2021 Aug 16;40(16):e107911

Publication year

2021

Abstract

Cell growth is orchestrated by a number of interlinking cellular processes. Components of the TOR pathway have been proposed as potential regulators of cell growth, but little is known about their immediate effects on protein synthesis in response to TOR-dependent growth inhibition. Here, we present a resource providing an in-depth characterisation of Schizosaccharomyces pombe phosphoproteome in relation to changes observed in global cellular protein synthesis upon TOR inhibition. We find that after TOR inhibition, the rate of protein synthesis is rapidly reduced and that notable phosphorylation changes are observed in proteins involved in a range of cellular processes. We show that this reduction in protein synthesis rates upon TOR inhibition is not dependent on S6K activity, but is partially dependent on the S. pombe homologue of eIF4G, Tif471. Our study demonstrates the impact of TOR-dependent phospho-regulation on the rate of protein synthesis and establishes a foundational resource for further investigation of additional TOR-regulated targets both in fission yeast and other eukaryotes.

Annotation