Reference - PMID:34504165 - Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast.
Reference summary
- PubMed ID
- PMID:34504165
- Title
- Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast.
- Authors
- García P, Coll PM, Del Rey F, Geli MI, Pérez P, Vázquez de Aldana CR, Encinar Del Dedo J
- Citation
- Sci Rep 2021 Sep 09;11(1):17872
- Publication year
- 2021
- Abstract
- Cell polarity and morphogenesis are regulated by the small GTPase Cdc42. Even though major advances have been done in the field during the last years, the molecular details leading to its activation in particular cellular contexts are not completely understood. In fission yeast, the β(1,3)-glucanase Eng2 is a "moonlighting protein" with a dual function, acting as a hydrolase during spore dehiscence, and as component of the endocytic machinery in vegetative cells. Here, we report that Eng2 plays a role in Cdc42 activation during polarized growth through its interaction with the scaffold protein Scd2, which brings Cdc42 together with its guanine nucleotide exchange factor (GEF) Scd1. eng2Δ mutant cells have defects in activation of the bipolar growth (NETO), remaining monopolar during all the cell cycle. In the absence of Eng2 the accumulation of Scd1 and Scd2 at the poles is reduced, the levels of Cdc42 activation decrease, and the Cdc42 oscillatory behavior, associated with bipolar growth in wild type cells, is altered. Furthermore, overexpression of Eng2 partially rescues the growth and polarity defects of a cdc42-L160S mutant. Altogether, our work unveils a new factor regulating the activity of Cdc42, which could potentially link the polarity and endocytic machineries.
