Reference - PMID:34974803 - Rrp14 controls rRNA transcription via facilitating the translocation of Pol5 into the nucleolus.
Reference summary
- PubMed ID
- PMID:34974803
- Title
- Rrp14 controls rRNA transcription via facilitating the translocation of Pol5 into the nucleolus.
- Authors
- Lin Z, Liu H, Chen H, Cao H, Liu X, Zhu H, Zhao L, Chen Z
- Citation
- Cell Cycle 2022 Mar;21(5):489-500
- Publication year
- 2022
- Abstract
- Rrp14 is a conserved protein that plays an important role in rRNA processing and ribosomal biogenesis. In Schizosaccharomyces pombe , the rrp14 gene is split into SPAC8C9.10 c ( rrp14 ) and SPBC947.07 ( rrp1402 ). Although the SPAC8C9.10 c gene is not essential for S. pombe survival, deletion of the gene causes the yeast cells to grow sick and to exhibit decreased rRNA transcription. We identified a novel Pol5 protein that physically interacts with the Rrp14 protein. Taking advantage of the Pil1 co-tethering assay, we found that Rrp14 facilitates the nucleolus translocation of Pol5, and the 7-RINAWN-12 motif of the Rrp14 protein is responsible for the interaction between Pol5 and Rrp14. Since deletion of the 7-RINAWN-12 motif affects rRNA transcription, we thus propose that Rrp14 affects rRNA transcription by facilitating the nucleolus translocation of Pol5.
