Reference - PMID:35300005 - Canavanine resistance mutation can1-1 in Schizosaccharomyces pombe is a missense mutation in the ubiquitin ligase adaptor gene any1 .
Reference summary
- PubMed ID
- PMID:35300005
- Title
- Canavanine resistance mutation can1-1 in Schizosaccharomyces pombe is a missense mutation in the ubiquitin ligase adaptor gene any1 .
- Authors
- Yang YS, Ning SK, Lyu XH, Suo F, Jia GS, Li W, Du LL
- Citation
- MicroPubl Biol 2022;2022
- Publication year
- 2022
- Abstract
- In Schizosaccharomyces pombe , the can1-1 mutation confers resistance to the toxic arginine analog canavanine. This mutation has been assumed to disrupt a gene encoding an arginine transporter. In PomBase, the gene SPBC18H10.16 is currently designated can1 . Here, we sequenced the genomes of three can1-1 strains. No mutations were found in SPBC18H10.16 . Instead, these strains harbor an R175C mutation in the gene any1 ( SPBC18H10.20c ). any1 encodes an α-arrestin that acts as a ubiquitin ligase adaptor to downregulate plasma membrane amino acid transporters. Our findings indicate that can1-1 is not a loss-of-function mutation in an amino acid transporter gene, but a possible gain-of-function mutation in a gene encoding a negative regulator of amino acid transporters.
