Reference - PMID:37191320 - Hva22, a REEP family protein in fission yeast, promotes reticulophagy in collaboration with a receptor protein.
Reference summary
- PubMed ID
- PMID:37191320
- Title
- Hva22, a REEP family protein in fission yeast, promotes reticulophagy in collaboration with a receptor protein.
- Authors
- Fukuda T, Saigusa T, Furukawa K, Inoue K, Yamashita SI, Kanki T
- Citation
- Autophagy 2023 Oct;19(10):2657-2667
- Publication year
- 2023
- Abstract
- The endoplasmic reticulum (ER) undergoes selective autophagy called reticulophagy or ER-phagy. Multiple reticulon- and receptor expression enhancing protein (REEP)-like ER-shaping proteins, including budding yeast Atg40, serve as reticulophagy receptors that stabilize the phagophore on the ER by interacting with phagophore-conjugated Atg8. Additionally, they facilitate phagophore engulfment of the ER by remodeling ER morphology. We reveal that Hva22, a REEP family protein in fission yeast, promotes reticulophagy without Atg8-binding capacity. The role of Hva22 in reticulophagy can be replaced by expressing Atg40 independently of its Atg8-binding ability. Conversely, adding an Atg8-binding sequence to Hva22 enables it to substitute for Atg40 in budding yeast. Thus, the phagophore-stabilizing and ER-shaping activities, both of which Atg40 solely contains, are divided between two separate factors, receptors and Hva22, respectively, in fission yeast. Abbreviations: AIM: Atg8-family interacting motif; Atg: autophagy related; DTT: dithiothreitol; ER: endoplasmic reticulum GFP: green fluorescent protein; NAA: 1-naphthaleneacetic acid; REEP: receptor expression enhancing protein; RFP: red fluorescent protein; UPR: unfolded protein response.
