Reference - PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Reference summary
- PubMed ID
- PMID:37970674
- Title
- SUMOylation regulates Lem2 function in centromere clustering and silencing.
- Authors
- Strachan J, Leidecker O, Spanos C, Le Coz C, Chapman E, Arsenijevic A, Zhang H, Zhao N, Spoel SH, Bayne EH
- Citation
- J Cell Sci 2023 Dec 01;136(23)
- Publication year
- 2023
- Abstract
- Regulation by the small modifier SUMO is heavily dependent on spatial control of enzymes that mediate the attachment and removal of SUMO on substrate proteins. Here, we show that in the fission yeast Schizosaccharomyces pombe, delocalisation of the SUMO protease Ulp1 from the nuclear envelope results in centromeric defects that can be attributed to hyper-SUMOylation at the nuclear periphery. Unexpectedly, we find that although this localised hyper-SUMOylation impairs centromeric silencing, it can also enhance centromere clustering. Moreover, both effects are at least partially dependent on SUMOylation of the inner nuclear membrane protein Lem2. Lem2 has previously been implicated in diverse biological processes, including the promotion of both centromere clustering and silencing, but how these distinct activities are coordinated was unclear; our observations suggest a model whereby SUMOylation serves as a regulatory switch, modulating Lem2 interactions with competing partner proteins to balance its roles in alternative pathways. Our findings also reveal a previously unappreciated role for SUMOylation in promoting centromere clustering.
Annotation
Multi-locus phenotype
Genes:
Genotypes:
- bqt4delta csi1delta
- csi1delta lem2-K7R(K50R,K64R,K72R,K83R,K92R,K121R,K150R aa)
- csi1delta lem2-K7R(K50R,K64R,K72R,K83R,K92R,K121R,K150R aa) nup132delta
- csi1delta nup132delta
- csi1delta nup132delta pli1delta
- csi1delta nup132delta pmt3+(wild type)[Overexpression]
- csi1delta nup132delta pmt3-KallR(K14R,K30R,K39R,K51R,K53R,K54R,K60R,K63R,K71R aa)[Overexpression]
- csi1delta nup132delta slx8delta
- csi1delta nup132delta ufd1deltaCt(213-342 Δaa)
- csi1delta nup132delta ulp1+(wild type)[Overexpression]
- csi1delta pli1delta
- csi1delta pmt3+(wild type)[Overexpression]
- csi1delta pmt3-KallR(K14R,K30R,K39R,K51R,K53R,K54R,K60R,K63R,K71R aa)[Overexpression]
- csi1delta slx8delta
- csi1delta ufd1deltaCt(213-342 Δaa)
- csi1delta ulp1+(wild type)[Overexpression]
- lem2-K7R(K50R,K64R,K72R,K83R,K92R,K121R,K150R aa) nup132delta
- nup132delta pli1delta
- nup132delta pmt3+(wild type)[Overexpression]
- nup132delta pmt3-KallR(K14R,K30R,K39R,K51R,K53R,K54R,K60R,K63R,K71R aa)[Overexpression]
- nup132delta slx8delta
- nup132delta ufd1deltaCt(213-342 Δaa)
- nup132delta ulp1+(wild type)[Overexpression]
Genes:
Genotypes:
- csi1delta lem2-K7R(K50R,K64R,K72R,K83R,K92R,K121R,K150R aa)
- csi1delta lem2-K7R(K50R,K64R,K72R,K83R,K92R,K121R,K150R aa) nup132delta
- csi1delta nup132delta
Genes:
Genotypes:
- lem2-K7R(K50R,K64R,K72R,K83R,K92R,K121R,K150R aa) nup132delta
- lem2-LuC(1-308 Δaa) nup132delta
- nup107-ulp1(GFP-Nup107-HM-Ulp1 fusion) nup132delta
- nup132delta pli1-K3R(K15R,K169R,K214R aa)
- nup132delta pmt3-KallR(K14R,K30R,K39R,K51R,K53R,K54R,K60R,K63R,K71R aa)[Overexpression]
- nup132delta ulp1+(wild type)[Overexpression]
Single locus phenotype
Genes:
Genotypes:
- lem2-K7R(K50R,K64R,K72R,K83R,K92R,K121R,K150R aa)
- lem2-LuC(1-308 Δaa)
- nup107-ulp1(GFP-Nup107-HM-Ulp1 fusion)
- pli1-K3R(K15R,K169R,K214R aa)
- pmt3-KallR(K14R,K30R,K39R,K51R,K53R,K54R,K60R,K63R,K71R aa)[Overexpression]
- ulp1+(wild type)[Overexpression]
