Reference - PMID:38448160 - Phosphorylation of Rec8 cohesin complexes regulates mono-orientation of kinetochores in meiosis I.

Reference summary

PubMed ID

PMID:38448160

Title

Phosphorylation of Rec8 cohesin complexes regulates mono-orientation of kinetochores in meiosis I.

Authors

Liu Y, Min Y, Liu Y, Watanabe Y

Citation

Life Sci Alliance 2024 May;7(5)

Publication year

2024

Abstract

In meiosis I, unlike in mitosis, sister kinetochores are captured by microtubules emanating from the same spindle pole (mono-orientation) and centromeric cohesion mediated by cohesin is protected in the following anaphase I. The conserved meiosis-specific kinetochore protein meikin (Moa1 in fission yeast) associates with polo-like kinase: Plo1 and regulates both mono-orientation and cohesion protection. Although the phosphorylation of Rec8-S450 by Plo1 associated with Moa1 plays a key role in cohesion protection, how Moa1-Plo1 regulates mono-orientation remains elusive. Here, we identify Plo1 phosphorylation sites in the cohesin subunits, Rec8 and Psm3. The non-phosphorylatable mutations at these sites showed specific defects in mono-orientation. These results enabled the genetic dissection of meikin functions at the centromeres.

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