Reference - PMID:39477503 - A DUF3844 domain-containing protein is required for vacuolar protein sorting in Schizosaccharomyces pombe.
Reference summary
- PubMed ID
- PMID:39477503
- Title
- A DUF3844 domain-containing protein is required for vacuolar protein sorting in Schizosaccharomyces pombe.
- Authors
- Inagawa T, Ohkubo K, Watanabe M, Morita T, Higuchi Y, Maekawa H, Takegawa K
- Citation
- J Gen Appl Microbiol 2024 Oct 31;
- Publication year
- 2024
- Abstract
- Protein trafficking to vacuoles in plants and fungi, and to lysosomes in animals, is essential for the maintenance of cellular homeostasis. In Saccharomyces cerevisiae, the vacuolar protein sorting (VPS) pathway has been well studied by using vacuolar carboxypeptidase Y (CPY) as a model, and many VPS genes have been identified. By contrast, the vacuolar protein trafficking pathway in Schizosaccharomyces pombe remains poorly understood. In this study, we identified a novel VPS gene (SPBC1709.03) in S. pombe that is broadly conserved in fungi, but not in S. cerevisiae. Owing to its DUF3844 domain of unknown function, the gene was named vps3844. Disruption mutants of vps3844 had defects in both CPY sorting and incorporation of FM4-64 dye into the vacuolar membrane. Partial deletion analysis of the Vps3844 protein revealed that, within the DUF3844 domain, the region comprising amino acids 354 to 380 is important for protein trafficking to the vacuole. Our findings represent the first report of a VPS gene involved in vacuolar transport that is conserved in fungi, particularly S. pombe, but lacks representation in S. cerevisiae.
