Reference - PMID:41676715 - Components of an ESCRT-independent nuclear envelope assembly pathway.
Reference summary
- PubMed ID
- PMID:41676715
- Title
- Components of an ESCRT-independent nuclear envelope assembly pathway.
- Authors
- Sydir EM, Farra MH, Whitford AL, Hinojosa S, Kao PY, Paulo JA, Swarup S, Lusk CP, Harper JW, Lee IJ, Pellman D
- Citation
- bioRxiv 2026 Feb 03;
- Publication year
- 2026
- Abstract
- Following chromosome segregation, the nuclear envelope (NE) must be reassembled and holes in the nuclear membrane must be "sealed." During NE assembly, the NE-specific adaptor, Cmp7, recruits/activates ESCRT-III proteins to mediate NE sealing. However, recent evidence suggests the presence of alternative mechanisms. In a screen using the fission yeast, S. japonicus , we recently implicated the ESCRT adaptor, Alx1, and a conserved, but little studied protein, Vid27, in Cmp7-independent NE assembly. Here, we provide direct evidence that Alx1 functions in a Cmp7- and ESCRT-independent NE assembly pathway via positive regulation of Vid27. Consistent with a role in membrane remodeling, Vid27 localizes to sites of postmitotic NE sealing and is essential in S. japonicus . Alx1 and Vid27 form a complex and mutations disrupting their interaction abolish Alx1's enhancement of Vid27 function at the NE. These findings define components of a new Cmp7- and ESCRT-independent NE assembly pathway, advancing our understanding of the mechanisms crucial for maintaining the integrity of the nucleus.
