Reference - PMID:7501024 - Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast.
Reference summary
- PubMed ID
- PMID:7501024
- Title
- Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast.
- Authors
- Shiozaki K, Russell P
- Citation
- Nature 1995 Dec 14;378(6558):739-43
- Publication year
- 1995
- Abstract
- In fission yeast the onset of mitosis is brought about by Cdc2/Cdc13 kinase, which is inhibited by the Wee1/Mik1 tyrosine kinases and activated by Cdc25 tyrosine phosphatase. This control network integrates many signals, including those that monitor DNA replication, DNA damage and cell size. We report here that a fission yeast MAP kinase pathway links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Fission yeast spc1- mutants have a G2 delay that is greatly exacerbated by growth in high osmolarity media and nutrient limitation. A lethal interaction of spc1 and cdc25 mutations shows that Spc1 promotes the onset of mitosis. Spc1 is a MAP kinase homologue that is activated by Wis1 kinase in response to osmotic stress and nutrient limitation. Spc1 is inactivated by Pyp1, a phosphatase previously identified as a mitotic inhibitor. Pyp1 dephosphorylates only tyrosine-173 of Spc1, unlike the dual-specificity phosphatases that have been shown to regulate other MAP kinases.
