Reference - PMID:8211184 - U2AF homolog required for splicing in vivo.
Reference summary
- PubMed ID
- PMID:8211184
- Title
- U2AF homolog required for splicing in vivo.
- Authors
- Potashkin J, Naik K, Wentz-Hunter K
- Citation
- Science 1993 Oct 22;262(5133):573-5
- Publication year
- 1993
- Abstract
- Several fission yeast temperature-sensitive mutants defective in pre-mRNA processing (prp- mutants) at the nonpermissive temperature have been identified. Here, the prp2+ gene has been cloned by its ability to complement the temperature-sensitive growth defect of a prp2- mutant. The gene also corrects the pre-mRNA splicing defect of prp2- mutants and encodes a 59-kilodalton polypeptide (PRP2). A molecular characterization indicates that PRP2 is a previously uncharacterized yeast splicing factor with extensive similarity to the mammalian splicing factor U2AF65. Thus, this study provides evidence that a U2AF homolog participates in RNA processing in vivo.
