Reference - PMID:8247131 - Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit.
Reference summary
- PubMed ID
- PMID:8247131
- Title
- Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit.
- Authors
- Gordon C, McGurk G, Dillon P, Rosen C, Hastie ND
- Citation
- Nature 1993 Nov 25;366(6453):355-7
- Publication year
- 1993
- Abstract
- We have isolated a mutant, mts2, in the fission yeast Schizosaccharomyces pombe which is defective in chromosome segregation. The predicted amino-acid sequence of the cloned mts2+ gene product is 75% identical to the S4 subunit of the human 26S ATP/ubiquitin-dependent protease. The human S4 subunit complementary DNA expressed from an S. pombe expression plasmid can rescue an S. pombe mts2 gene disruption. Both observations demonstrate that the mts2+ gene is the S. pombe homologue of the human S4 subunit. In addition, we provide genetic evidence for a physical interaction between the S4 and the related S7 subunit in the 26S multiprotein protease. We show that polyubiquitin-conjugated proteins accumulate in the mts2 mutant at the restrictive temperature, demonstrating that the mutant has an in vivo defect in the ubiquitin-dependent proteolysis pathway. Finally, the phenotype for the mts2 mutant indicates that protein degradation by the 26S protease is essential not for entry into but for the completion of mitosis.
