Reference - PMID:8887647 - Fission yeast mal2+ is required for chromosome segregation.
Reference summary
- PubMed ID
- PMID:8887647
- Title
- Fission yeast mal2+ is required for chromosome segregation.
- Authors
- Fleig U, Sen-Gupta M, Hegemann JH
- Citation
- Mol Cell Biol 1996 Nov;16(11):6169-77
- Publication year
- 1996
- Abstract
- By a screen designed to isolate new fission yeast genes required for chromosome segregation, we have identified mal2+. The conditionally lethal mal2-1 allele gives rise to increased loss of a nonessential minichromosome at the permissive temperature and leads to severe missegregation of the chromosomes at the nonpermissive temperature. Cloning by complementation and subsequent sequence analysis revealed that mal2 is a novel protein with a mass of 34 kDa. Cells containing a mal2 null allele were inviable, indicating that mal2+ is an essential gene. Fusion of mal2 protein to the green fluorescent protein (GFP) showed that mal2 was predominantly localized in the nucleus. Sensitivity to microtubule-destabilizing drugs and strong genetic interactions with alpha1-tubulin suggest an interaction of the mal2 protein with the microtubule system. Spindle formation and elongation were not detectably affected in the mal2-1 mutant as determined by indirect immunofluorescence. However, anomalous chromosome movement on the spindle leading to aberrant distribution of the chromosomal material was observed.
