Reference - PMID:9161410 - A novel HSP70 gene of Schizosaccharomyces pombe that confers K-252a resistance.
Reference summary
- PubMed ID
- PMID:9161410
- Title
- A novel HSP70 gene of Schizosaccharomyces pombe that confers K-252a resistance.
- Authors
- Usui T, Yoshida M, Kasahara K, Honda A, Beppu T, Horinouchi S
- Citation
- Gene 1997 Apr 11;189(1):43-7
- Publication year
- 1997
- Abstract
- A new gene encoding a heat shock protein 70 family protein of Schizosaccharomyces pombe (Sp), named sks2+, was cloned as a weak suppressor for the K-252a-sensitive mutation, ucm1. The nucleotide sequence of sks2+ revealed an open reading frame of a 613-amino-acid (aa) protein. The deduced aa sequence of sks2+ showed significant homology with Saccharomyces cerevisiae (Sc) Ssb1p and Ssb2p responsible for protein synthesis by non-organelle-localized ribosomes, as well as with other proteins of the HSP70 family. The cells lacking the functional sks2+ gene were viable and showed no increased sensitivity to K-252a but grew slowly with an elongated morphology. These results suggest that the sks2+ gene product plays a role in the cell cycle progression and is able to confer drug resistance in a multicopy state.
