Reference - PMID:9236781 - The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor.

Reference summary

PubMed ID

PMID:9236781

Title

The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor.

Authors

Christensen PU, Davey J, Nielsen O

Citation

Mol Gen Genet 1997 Jun;255(2):226-36

Publication year

1997

Abstract

In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

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