Reference - PMID:9301023 - Advancement through mitosis requires rae1 gene function in fission yeast.
Reference summary
- PubMed ID
- PMID:9301023
- Title
- Advancement through mitosis requires rae1 gene function in fission yeast.
- Authors
- Whalen WA, Bharathi A, Danielewicz D, Dhar R
- Citation
- Yeast 1997 Sep 30;13(12):1167-79
- Publication year
- 1997
- Abstract
- Growth of the rae1-1 mutant of Schizosaccharomyces pombe at restrictive temperature results in accumulation of poly(A)+ RNA in the nucleus and a cell cycle arrest at the G2/M boundary. We demonstrate here that rae1 function is required for a process other than mRNA export which is essential for advancement through mitosis. Cells lacking rae1 function arrest with elevated Cdc2p kinase levels at a step before the formation of a mitotic spindle and without separation of the spindle pole bodies. Rae1p was localized to the nuclear periphery, consistent with a role in nucleocytoplasmic trafficking, which could include protein import. We propose a model where rae1 functions in cell cycle progression through trafficking of proteins required for mitosis.
