Reference - PMID:9572736 - Replication checkpoint enforced by kinases Cds1 and Chk1.

Reference summary

PubMed ID

PMID:9572736

Title

Replication checkpoint enforced by kinases Cds1 and Chk1.

Authors

Boddy MN, Furnari B, Mondesert O, Russell P

Citation

Science 1998 May 08;280(5365):909-12

Publication year

1998

Abstract

Cdc2, the kinase that induces mitosis, is regulated by checkpoints that couple mitosis to the completion of DNA replication and repair. The repair checkpoint kinase Chk1 regulates Cdc25, a phosphatase that activates Cdc2. Effectors of the replication checkpoint evoked by hydroxyurea (HU) are unknown. Treatment of fission yeast with HU stimulated the kinase Cds1, which appears to phosphorylate the kinase Wee1, an inhibitor of Cdc2. The protein kinase Cds1 was also required for a large HU-induced increase in the amount of Mik1, a second inhibitor of Cdc2. HU-induced arrest of cell division was abolished in cds1 chk1 cells. Thus, Cds1 and Chk1 appear to jointly enforce the replication checkpoint.

Annotation