Reference - PMID:9809416 - Mutation of Gly-444 inactivates the S. pombe malic enzyme.
Reference summary
- PubMed ID
- PMID:9809416
- Title
- Mutation of Gly-444 inactivates the S. pombe malic enzyme.
- Authors
- Viljoen M, van der Merwe M, Subden RE, van Vuuren HJ
- Citation
- FEMS Microbiol Lett 1998 Oct 15;167(2):157-62
- Publication year
- 1998
- Abstract
- A mutant malic enzyme gene, mae2-, was cloned from a strain of Schizosaccharomyces pombe that displayed almost no malic enzyme activity. Sequence analysis revealed only one codon-altering mutation, a guanine to adenine at nucleotide 1331, changing the glycine residue at position 444 to an aspartate residue. Gly-444 is located in Region H, previously identified as one of eight highly conserved regions in malic enzymes. We found that Gly-444 is absolutely conserved in 27 malic enzymes from various prokaryotic and eukaryotic sources, as well as in three bacterial malolactic enzymes investigated. The evolutionary conservation of Gly-444 suggests that this residue is important for enzymatic function.
