bfr1 (SPCC18B5.01c)

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Gene Ontology:
Phenotype:
- Single-allele:
  - Population
  - Cell
- Multi-allele:
  - Population
Target Of
Transcript
Protein Features
Modifications
Sequence
Gene Expression
Taxonomic Conservation
Orthologs
Interactions:
- Physical
- Genetic
External References
Literature

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Gene Standard Name	bfr1	Characterisation Status	published
Systematic ID	SPCC18B5.01c	Feature Type	protein coding
Synonyms	hba2, SPCPJ732.04c	Name Description
Product	brefeldin A transmembrane transporter Bfr1	Product Size	1530aa, 171.75 kDa
Genomic Location	Chromosome III, 715435-709302 (6134nt); CDS:714007-709415 (4593nt)

GO Molecular Function

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Term Name

Count

ATP binding

551

Annotation Extension	Evidence	With/From	Reference
	IEA	UniProtKB-KW:KW-0067	GO_REF:0000037

ATPase activity, coupled to transmembrane movement of substances

Annotation Extension	Evidence	With/From	Reference
	IEA	IPR005285	GO_REF:0000002

Term ID	Term Name	Evidence	With/From	Reference	Count
GO:0005524	ATP binding	IEA	UniProtKB-KW:KW-0067	GO_REF:0000037	551
GO:0042626	ATPase activity, coupled to transmembrane movement of substances	IEA	IPR005285	GO_REF:0000002	52

GO Biological Process

GO Slim Terms

transmembrane transport

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Term Name

Count

drug export

Annotation Extension	Evidence	With/From	Reference
	IEA	IPR005285	GO_REF:0000002

transmembrane transport

346

Annotation Extension	Evidence	With/From	Reference
	ISM	PFAM:PF06422	GO_REF:0000050

Term ID	Term Name	Evidence	With/From	Reference	Count
GO:0046618	drug export	IEA	IPR005285	GO_REF:0000002	1
GO:0055085	transmembrane transport	ISM	PFAM:PF06422	GO_REF:0000050	346

GO Cellular Component

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Term Name

Count

integral component of membrane

969

Annotation Extension	Evidence	With/From	Reference
	IEA	UniProtKB-KW:KW-0812	GO_REF:0000037

plasma membrane

256

Annotation Extension	Evidence	With/From	Reference
	IDA		PMID:16849797

Term ID	Term Name	Evidence	With/From	Reference	Count
GO:0016021	integral component of membrane	IEA	UniProtKB-KW:KW-0812	GO_REF:0000037	969
GO:0005886	plasma membrane	IDA		PMID:16849797	256

FYPO Single-Allele Phenotypes

Gene Deletion Viability: Viable

Population Phenotype

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Term Name

Genotypes

Count

normal growth on amphotericin B

bfr1+ (wild type)

normal growth on caffeine

bfr1Δ

normal growth on clotrimazole

bfr1+ (wild type)

normal growth on FR901464

bfr1Δ

normal growth on mevastatin

bfr1+ (wild type)

normal growth on miconazole

bfr1+ (wild type)

normal growth on oligomycin

bfr1Δ

normal growth on staurosporine

bfr1Δ

resistance to actinomycin D

bfr1+ (wild type)

resistance to anisomycin

bfr1+ (wild type)

resistance to brefeldin A

bfr1+ (wild type)

resistance to cerulenin

bfr1+ (wild type)

resistance to clotrimazole

bfr1+ (wild type)

resistance to cycloheximide

bfr1+ (wild type)

resistance to cytochalasin B

bfr1+ (wild type)

resistance to K-252a

bfr1+ (wild type)

resistance to staurosporine

bfr1+ (wild type)

resistance to tamoxifen

bfr1+ (wild type)

resistance to terbinafine

bfr1+ (wild type)

sensitive to actinomycin D

bfr1Δ

sensitive to brefeldin A

bfr1Δ

sensitive to cerulenin

bfr1Δ

sensitive to clotrimazole

bfr1Δ

108

sensitive to cycloheximide

bfr1Δ

sensitive to cytochalasin B

bfr1Δ

sensitive to fluconazole

bfr1Δ

sensitive to G418

bfr1Δ

sensitive to hygromycin B

bfr1Δ

sensitive to K-252a

bfr1Δ

sensitive to miconazole

bfr1Δ

sensitive to MTT

bfr1Δ

sensitive to myriocin during vegetative growth

bfr1Δ

sensitive to purvalanol A

bfr1Δ

sensitive to staurosporine

bfr1Δ

sensitive to terbinafine

bfr1Δ

103

sensitive to tributyltin

bfr1Δ

sensitive to trichostatin A

bfr1Δ

sensitive to tunicamycin

bfr1Δ

sensitive to wortmannin

bfr1Δ

viable vegetative cell population

bfr1Δ

3862

Cell Phenotype

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Term Name

Genotypes

Count

increased pre-mRNA level

affecting fet5

bfr1Δ

viable vegetative cell with normal cell morphology

bfr1Δ

3103

FYPO Multi-allele Phenotypes

Population Phenotype

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Term Name

Genotypes

normal growth on cadmium

bfr1Δ, pdr1Δ

resistance to caffeine

trr1Δ, bfr1Δ

sensitive to brefeldin A

bfr1Δ, pmd1Δ

sensitive to caffeine during vegetative growth

bfr1Δ, trr1Δ, caf5Δ

sensitive to cycloheximide

bfr1Δ, pmd1Δ

sensitive to etoposide

bfr1Δ, pmd1Δ

sensitive to fluconazole

bfr1Δ, pmd1Δ

sensitive to FR901464

bfr1Δ, pmd1Δ

sensitive to G418

bfr1Δ, pmd1Δ

sensitive to hygromycin B

bfr1Δ, pmd1Δ

sensitive to leptomycin B

bfr1Δ, pmd1Δ

sensitive to lovastatin

bfr1Δ, pmd1Δ

sensitive to mevastatin

bfr1Δ, pmd1Δ

sensitive to MTT

bfr1Δ, pmd1Δ

sensitive to myriocin during vegetative growth

bfr1Δ, pmd1Δ

sensitive to radicicol

bfr1Δ, pmd1Δ

sensitive to staurosporine

bfr1Δ, pmd1Δ

sensitive to streptothricin

bfr1Δ, pmd1Δ

sensitive to trichostatin A

bfr1Δ, pmd1Δ

sensitive to tunicamycin

bfr1Δ, pmd1Δ

sensitive to wortmannin

bfr1Δ, pmd1Δ

Target Of

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Ontology	Relationship	Gene	Product
FYPO	affected by mutation in	atf1	transcription factor, Atf-CREB family Atf1
FYPO	affected by mutation in	crm1	importin family nuclear export receptor Crm1
FYPO	affected by mutation in	pap1	transcription factor Pap1/Caf3
FYPO	affected by mutation in	prt1	transcription factor (predicted)
FYPO	affected by mutation in	sty1	MAP kinase Sty1
GO	regulated by	atf1	transcription factor, Atf-CREB family Atf1
GO	regulated by	pap1	transcription factor Pap1/Caf3
GO	regulated by	prt1	transcription factor (predicted)
GO	regulated by	sty1	MAP kinase Sty1
GO	substrate of	sst2	human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO	substrate of	ubp15	ubiquitin C-terminal hydrolase Ubp15
GO	substrate of	ubp4	ubiquitin C-terminal hydrolase Ubp4
GO	substrate of	ubp5	ubiquitin C-terminal hydrolase Ubp5
GO	substrate of	ubp9	ubiquitin C-terminal hydrolase Ubp9

Transcript

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Transcript Structure

Region	Coordinates	Reference
Exons	715435..709302
mRNA	715435..709302
5' UTR	715435..714008	PMID:21511999
CDS	714007..709415
3' UTR	709414..709302	AU011775

Protein Features

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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID	Database	InterPro	Description	Start	End	Count
PF14510	Pfam	IPR029481	ABC-transporter extracellular N-terminal domain	79	158	1
PF06422	Pfam	IPR010929	CDR ABC transporter	742	831	2
PF06422	Pfam	IPR010929	CDR ABC transporter	1473	1514	2
PF01061	Pfam	IPR013525	ABC-2 type transporter	521	731	2
PF01061	Pfam	IPR013525	ABC-2 type transporter	1202	1413	2
PF00005	Pfam	IPR003439	ABC transporter-like	902	1052	19
PF00005	Pfam	IPR003439	ABC transporter-like	185	336	19
SM00382	SMART	IPR003593	AAA+ ATPase domain	910	1110	70
TMhelix	TMHMM			619	641	950
TMhelix	TMHMM			1490	1512	950
TMhelix	TMHMM			573	595	950
TMhelix	TMHMM			1290	1323	950
TMhelix	TMHMM			1253	1275	950
TMhelix	TMHMM			648	670	950
TMhelix	TMHMM			1336	1358	950
TMhelix	TMHMM			787	809	950
TMhelix	TMHMM			538	560	950
TMhelix	TMHMM			685	707	950
TMhelix	TMHMM			1218	1240	950
TMhelix	TMHMM			1373	1395	950
PS50893	Prosite Profiles	IPR003439	ABC transporter-like	882	1125	19
PS50893	Prosite Profiles	IPR003439	ABC transporter-like	153	410	19
PS00211	Prosite Patterns	IPR017871	ABC transporter, conserved site	309	323	14
PTHR19241	HMMPANTHER			220	473	2
PTHR19241	HMMPANTHER			494	750	2
PTHR19241:SF299	HMMPANTHER			220	473	2
PTHR19241	HMMPANTHER			1457	1521	2
PTHR19241	HMMPANTHER			854	1428	2
PTHR19241:SF299	HMMPANTHER			1457	1521	2
PTHR19241:SF299	HMMPANTHER			854	1428	2
PTHR19241:SF299	HMMPANTHER			494	750	2
3.40.50.300	Gene3D	IPR027417	P-loop containing nucleoside triphosphate hydrolase	173	399	288
3.40.50.300	Gene3D	IPR027417	P-loop containing nucleoside triphosphate hydrolase	883	1122	288
SSF52540	SuperFamily	IPR027417	P-loop containing nucleoside triphosphate hydrolase	174	401	294
SSF52540	SuperFamily	IPR027417	P-loop containing nucleoside triphosphate hydrolase	888	1112	294
TIGR00956	TIGRFAM	IPR005285	Pleiotropic drug resistance protein PDR/CDR	108	1516	1

View domain organization at Pfam

Term ID	Term Name	Reference	Count
PBO:0001765	ABC transporter family
PBO:0001815	PDR subfamily

Term ID	Term Name	Reference	Count
SO:0001812	transmembrane_helix		465

Protein Properties

Ave. residue weight	112.26 Da
Charge	-4.00
Codon Adaptation Index	0.50
Isoelectric point	6.32
Molecular weight	171.75 kDa
Number of residues	1530

Modifications

Protein Modifications

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Term Name

Residue

Count

O-phospho-L-serine

2293

present during mitotic M phase

S489

present during mitotic M phase

S475

present during mitotic M phase

S861

present during mitotic M phase

S486

present during mitotic M phase

S64

present during mitotic M phase

S476

present during mitotic M phase

S56

present during mitotic M phase

S1188

S27, S29, S30, S36, S37, S38, S56, S475, S476, S486, S1188

Annotation Extension	Evidence	Residue	Reference
present during mitotic M phase	experimental evidence	S489	PMID:21712547
present during mitotic M phase	experimental evidence	S475	PMID:21712547
present during mitotic M phase	experimental evidence	S861	PMID:21712547
present during mitotic M phase	experimental evidence	S486	PMID:21712547
present during mitotic M phase	experimental evidence	S64	PMID:21712547
present during mitotic M phase	experimental evidence	S476	PMID:21712547
present during mitotic M phase	experimental evidence	S56	PMID:21712547
present during mitotic M phase	experimental evidence	S1188	PMID:21712547
	IDA	S37	PMID:25720772
	IDA	S29	PMID:25720772
	IDA	S486	PMID:25720772
	IDA	S36	PMID:25720772
	experimental evidence	S475	PMID:24763107
	IDA	S30	PMID:25720772
	experimental evidence	S476	PMID:24763107
	experimental evidence	S486	PMID:24763107
	IDA	S38	PMID:25720772
	IDA	S27	PMID:25720772
	experimental evidence	S56	PMID:24763107
	IDA	S1188	PMID:25720772

O-phospho-L-threonine

1087

present during mitotic M phase

T58

present during mitotic M phase

T471

present during mitotic M phase

T472

present during mitotic M phase

T492

present during mitotic M phase

T1186

level fluctuates during mitotic cell cycle

T472

Annotation Extension	Evidence	Residue	Reference
present during mitotic M phase	experimental evidence	T58	PMID:21712547
present during mitotic M phase	experimental evidence	T471	PMID:21712547
present during mitotic M phase	experimental evidence	T472	PMID:21712547
present during mitotic M phase	experimental evidence	T492	PMID:21712547
present during mitotic M phase	experimental evidence	T1186	PMID:21712547
level fluctuates during mitotic cell cycle	experimental evidence	T472	PMID:24763107

phosphorylated residue

2515

Annotation Extension	Evidence	Residue	Reference
	IDA		PMID:19547744

sumoylated lysine

174

Annotation Extension	Evidence	Residue	Reference
	IDA		PMID:26404184

O-phosphorylated residue

S27,S29, S27,S29,S36, S27,S30, S27,S36, S29,S30, S29,S36,S37, S29,S38, S30,S38, S36,S37, S36,S38, S37,S38, S475,S476,S486, S476,S486,S489, S486,S489, S486,T491, S486,T492

2461

Evidence	Residue	Reference
IDA	S29,S30	PMID:25720772
IDA	S30,S38	PMID:25720772
IDA	S486,T491	PMID:25720772
IDA	S27,S30	PMID:25720772
IDA	S486,T492	PMID:25720772
IDA	S36,S38	PMID:25720772
IDA	S29,S38	PMID:25720772
IDA	S27,S29	PMID:25720772
IDA	S27,S36	PMID:25720772
IDA	S37,S38	PMID:25720772
IDA	S27,S29,S36	PMID:25720772
IDA	S475,S476,S486	PMID:25720772
IDA	S29,S36,S37	PMID:25720772
IDA	S476,S486,S489	PMID:25720772
IDA	S36,S37	PMID:25720772
IDA	S486,S489	PMID:25720772

Sequence

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Gene Expression

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Qualitative Gene Expression

Description	Level	Evidence	Reference
RNA level	increased in presence of purvalanol A	expression microarray evidence	PMID:22840777
	increased during GO:0070301	Northern assay evidence	PMID:9585505
	present during GO:0072690	reverse transcription polymerase chain reaction transcription evidence	PMID:22840777
	increased during GO:0034599	expression microarray evidence	PMID:12529438
	increased in presence of purvalanol A	reverse transcription polymerase chain reaction transcription evidence	PMID:22840777
	present during GO:0072690	Northern assay evidence	PMID:9532803
	present during GO:0072690	Northern assay evidence	PMID:9585505
	increased during GO:0071409	reverse transcription polymerase chain reaction transcription evidence	PMID:22840777

Quantitative Gene Expression

View graphical display of gene expression data for bfr1 (SPCC18B5.01c)

Protein Level

Molecules/Cell (average)	Extension	Condition	Scale	Evidence	Reference
17011	during GO:0000080	PECO:0000005, PECO:0000126	single cell	mass spectrometry evidence	PMID:24763107
15946	during GO:0000084	PECO:0000005, PECO:0000126	single cell	mass spectrometry evidence	PMID:24763107
17353	during GO:0000085	PECO:0000005, PECO:0000126	single cell	mass spectrometry evidence	PMID:24763107
16023	during GO:0000087	PECO:0000005, PECO:0000126	single cell	mass spectrometry evidence	PMID:24763107
17758	during GO:0072690	PECO:0000005, PECO:0000126	single cell	mass spectrometry evidence	PMID:24763107
11505.73	during GO:0072690	PECO:0000126, PECO:0000005	population wide	experimental evidence	PMID:23101633
6503.26	during cell quiescence following G1 arrest due to nitrogen limitation	PECO:0000127, PECO:0000126, PECO:0000005	population wide	experimental evidence	PMID:23101633

RNA Level

Molecules/Cell (average)	Extension	Condition	Scale	Evidence	Reference
16	during GO:0072690	PECO:0000126, PECO:0000005	population wide	experimental evidence	PMID:23101633
3.3	during cell quiescence following G1 arrest due to nitrogen limitation	PECO:0000127, PECO:0000126, PECO:0000005	population wide	experimental evidence	PMID:23101633

Taxonomic Conservation

Description	Qualifier	Reference	Count
conserved in fungi			4608
conserved in eukaryotes			4516

Orthologs

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Manually curated orthologous groups

Species	Gene	Description	Links	Count

Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC18B5.01c into the Query Builder
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View the HCPIN interactions in esyN

	Gene	Product	Evidence	Reference
affinity captured by	imp1	importin alpha	Affinity Capture-MS	PMID:25963819
affinity captured by	sdj1	glyoxylase III sdj1	Affinity Capture-MS	PMID:26152728

Genetic Interactions

Source: BioGRID

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	Gene	Product	Evidence	Reference
synthetic growth defect with	pap1	transcription factor Pap1/Caf3	Synthetic Growth Defect	PMID:22840777
synthetic growth defect with	mfs1	transmembrane transporter Mfs1	Synthetic Growth Defect	PMID:22840777
synthetic growth defect with	mfs3	transmembrane transporter Mfs3	Synthetic Growth Defect	PMID:22840777
synthetic growth defect with	prt1	transcription factor (predicted)	Synthetic Growth Defect	PMID:22840777
synthetic growth defect with	pmd1	leptomycin transmembrane transporter Pmd1	Synthetic Growth Defect	PMID:21340088
synthetically rescued by	mfs3	transmembrane transporter Mfs3	Synthetic Rescue	PMID:22840777
synthetically rescued by	trr1	thioredoxin reductase Trr1	Synthetic Rescue	PMID:19672306
negative genetic interaction with	prt1	transcription factor (predicted)	Negative Genetic	PMID:22840777
negative genetic interaction with	mfs3	transmembrane transporter Mfs3	Negative Genetic	PMID:22840777
negative genetic interaction with	mfs1	transmembrane transporter Mfs1	Negative Genetic	PMID:22840777
positive genetic interaction with	mfs3	transmembrane transporter Mfs3	Positive Genetic	PMID:22840777

External References

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Database	Identifier	Description
NBRP	SPCC18B5.01c	Fission yeast strain database, National BioResource Project (Japan)
YOGY	SPCC18B5.01c	Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid	SPCC18B5.01c	BioGRID Interaction Datasets
Expression Viewer	SPCC18B5.01c	Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer	SPCC18B5.01c	Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer	SPCC18B5.01c	Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer	SPCC18B5.01c	Environmental stress expression profiles (Bähler Lab)
Pomb(A)	SPCC18B5.01c	Polyadenylation Viewer (Gullerova lab)
pombeTV	SPCC18B5.01c	Transcriptome Viewer (Bähler Lab)
GEO	SPCC18B5.01c	GEO profiles
PInt	SPCC18B5.01c	Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas	SPCC18B5.01c	Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO	SPCC18B5.01c	Fission yeast phenotypic data & analysis
Cyclebase	SPCC18B5.01c.1	Cell Cycle Data
SPD / RIKEN	49/49H06	Orfeome Localization Data
UniProtKB/SwissProt	P41820	Brefeldin A resistance protein
ModBase	P41820	Database of comparative protein structure models
STRING	P41820	Network display of known and predicted interactions and functional associations
RefSeq Peptide	NP_587932	brefeldin A efflux transporter Bfr1
RefSeq mRNA	NM_001022923	972h- brefeldin A efflux transporter Bfr1 (bfr1), mRNA
European Nucleotide Archive	AAB33744.1	ENA Protein Mapping
European Nucleotide Archive	BAA19929.1	ENA Protein Mapping
European Nucleotide Archive	CAA58062.1	ENA Protein Mapping
European Nucleotide Archive	CAC34990.1	ENA Protein Mapping
UniParc	UPI000004C4D2	UniProt Archive

Literature for bfr1

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Release Version: PomBase:30_62 - 30 Jan 2017

Term ID	Term Name	Evidence	Genotype		Condition	Reference	Count
Term ID	Term Name	Evidence	Alleles	Expression	Condition	Reference	Count
FYPO:0000980	normal growth on amphotericin B						17
FYPO:0000980		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126	PMID:7646493	17
FYPO:0001522	normal growth on caffeine						14
FYPO:0001522		Cell growth assay	bfr1Δ	Null	PECO:0000137	PMID:19672306	14
FYPO:0002643	normal growth on clotrimazole						13
FYPO:0002643		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000142, PECO:0000201, PECO:0000005	PMID:26628015	13
FYPO:0003852	normal growth on FR901464						10
FYPO:0003852		Cell growth assay	bfr1Δ	Null		PMID:21340088	10
FYPO:0000978	normal growth on mevastatin						1
FYPO:0000978		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126	PMID:7646493	1
FYPO:0000979	normal growth on miconazole						12
FYPO:0000979		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126	PMID:7646493	12
FYPO:0003850	normal growth on oligomycin						10
FYPO:0003850		Cell growth assay	bfr1Δ	Null		PMID:21340088	10
FYPO:0001238	normal growth on staurosporine						8
FYPO:0001238		Cell growth assay	bfr1Δ	Null	PECO:0000126, PECO:0000103, PECO:0000167, PECO:0000005	PMID:9532803	8
FYPO:0000831	resistance to actinomycin D						4
		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000005, PECO:0000103, PECO:0000167, PECO:0000126	PMID:9532803
		Cell growth assay	bfr1+ (wild type)	Overexpression		PMID:7883711
FYPO:0000924	resistance to anisomycin						1
FYPO:0000924		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126	PMID:7646493	1
FYPO:0000067	resistance to brefeldin A						27
		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126	PMID:7646493
		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000005, PECO:0000103, PECO:0000167, PECO:0000126	PMID:9532803
		Cell growth assay	bfr1+ (wild type)	Overexpression		PMID:7883711
FYPO:0001941	resistance to cerulenin						1
FYPO:0001941		Cell growth assay	bfr1+ (wild type)	Overexpression		PMID:7883711	1
FYPO:0002766	resistance to clotrimazole						19
FYPO:0002766	expressivity FYPO_EXT:0000001	Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000005, PECO:0000201, PECO:0000142	PMID:26628015	19
FYPO:0000764	resistance to cycloheximide						6
FYPO:0000764		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126	PMID:7646493	6
FYPO:0001499	resistance to cytochalasin B						3
		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126, PECO:0000005, PECO:0000167, PECO:0000103	PMID:9532803
		Cell growth assay	bfr1+ (wild type)	Overexpression		PMID:7883711
FYPO:0000068	resistance to K-252a						4
FYPO:0000068		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000126, PECO:0000167, PECO:0000005, PECO:0000103	PMID:9532803	4
FYPO:0000767	resistance to staurosporine						16
FYPO:0000767		Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000167, PECO:0000103, PECO:0000005, PECO:0000126	PMID:9532803	16
FYPO:0005253	resistance to tamoxifen						61
FYPO:0005253	expressivity FYPO_EXT:0000002	Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000005, PECO:0000201, PECO:0000142	PMID:26628015	61
FYPO:0002767	resistance to terbinafine						14
FYPO:0002767	expressivity FYPO_EXT:0000001	Cell growth assay	bfr1+ (wild type)	Overexpression	PECO:0000005, PECO:0000201, PECO:0000142	PMID:26628015	14
FYPO:0000717	sensitive to actinomycin D						3
FYPO:0000717		Cell growth assay	bfr1Δ	Null	PECO:0000126, PECO:0000103, PECO:0000167, PECO:0000005	PMID:9532803	3
FYPO:0001501	sensitive to brefeldin A						16
		Cell growth assay	bfr1Δ	Null	PECO:0000126, PECO:0000005, PECO:0000167, PECO:0000103	PMID:9532803
		Cell growth assay	bfr1Δ	Null	PECO:0000137	PMID:24463365
		Cell growth assay	bfr1Δ	Null		PMID:21340088
		Cell growth assay	bfr1Δ	Null		PMID:7883711
		Cell growth assay	bfr1Δ	Null	PECO:0000137, PECO:0000102, PECO:0000005	PMID:22840777
		Cell growth assay	bfr1Δ	Null	PECO:0000005, PECO:0000137, PECO:0000102	PMID:16849797
FYPO:0002237	sensitive to cerulenin						11
FYPO:0002237		Cell growth assay	bfr1Δ	Null	PECO:0000005, PECO:0000137, PECO:0000102	PMID:16849797	11
FYPO:0002640	sensitive to clotrimazole						108
FYPO:0002640		Cell growth assay	bfr1Δ	Null	PECO:0000005, PECO:0000137, PECO:0000102	PMID:16849797	108
FYPO:0000104	sensitive to cycloheximide						31
		Cell growth assay	bfr1Δ	Null	PECO:0000102, PECO:0000137, PECO:0000005	PMID:22840777
		Cell growth assay	bfr1Δ	Null		PMID:21340088
		Cell growth assay	bfr1Δ	Null	PECO:0000005, PECO:0000137, PECO:0000102	PMID:16849797
FYPO:0001498	sensitive to cytochalasin B						2
FYPO:0001498		Cell growth assay	bfr1Δ	Null	PECO:0000103, PECO:0000005, PECO:0000167, PECO:0000126	PMID:9532803	2
FYPO:0003853	sensitive to fluconazole						4
FYPO:0003853		Cell growth assay	bfr1Δ	Null		PMID:21340088	4
FYPO:0000633	sensitive to G418						5
FYPO:0000633		Cell growth assay	bfr1Δ	Null		PMID:21340088	5
FYPO:0000106	sensitive to hygromycin B						16
FYPO:0000106		Cell growth assay	bfr1Δ	Null		PMID:21340088	16
FYPO:0000715	sensitive to K-252a						4
FYPO:0000715		Cell growth assay	bfr1Δ	Null	PECO:0000005, PECO:0000103, PECO:0000167, PECO:0000126	PMID:9532803	4
FYPO:0003358	sensitive to miconazole						40
FYPO:0003358		Cell growth assay	bfr1Δ	Null		PMID:21340088	40
FYPO:0003855	sensitive to MTT						1
FYPO:0003855		Cell growth assay	bfr1Δ	Null		PMID:21340088	1
FYPO:0003359	sensitive to myriocin during vegetative growth						2
FYPO:0003359		Cell growth assay	bfr1Δ	Null		PMID:21340088	2
FYPO:0001692	sensitive to purvalanol A						3
FYPO:0001692		Cell growth assay	bfr1Δ	Null	PECO:0000005, PECO:0000137, PECO:0000102	PMID:22840777	3
FYPO:0000113	sensitive to staurosporine						15
FYPO:0000113		Cell growth assay	bfr1Δ	Null		PMID:21340088	15
FYPO:0002328	sensitive to terbinafine						103
FYPO:0002328		Cell growth assay	bfr1Δ	Null		PMID:21340088	103
FYPO:0003718	sensitive to tributyltin						3
		Cell growth assay	bfr1Δ	Null	PECO:0000103, PECO:0000137	PMID:21297349
		Cell growth assay	bfr1Δ	Null	PECO:0000137, PECO:0000102	PMID:21297349
FYPO:0002546	sensitive to trichostatin A						44
FYPO:0002546		Cell growth assay	bfr1Δ	Null		PMID:21340088	44
FYPO:0001457	sensitive to tunicamycin						8
FYPO:0001457		Cell growth assay	bfr1Δ	Null		PMID:21340088	8
FYPO:0003859	sensitive to wortmannin						4
FYPO:0003859		Cell growth assay	bfr1Δ	Null		PMID:21340088	4
FYPO:0002060	viable vegetative cell population						3862
		Microscopy	bfr1Δ	Null	PECO:0000137, PECO:0000005	PMID:23697806
		Microscopy	bfr1Δ	Null		PMID:20473289
		Cell growth assay	bfr1Δ	Null	PECO:0000005, PECO:0000137, PECO:0000102	PMID:16849797

Term ID	Term Name	Evidence	Residue	Reference	Count
MOD:00046	O-phospho-L-serine				2293
	present during mitotic M phase	experimental evidence	S489	PMID:21712547
	present during mitotic M phase	experimental evidence	S475	PMID:21712547
	present during mitotic M phase	experimental evidence	S861	PMID:21712547
	present during mitotic M phase	experimental evidence	S486	PMID:21712547
	present during mitotic M phase	experimental evidence	S64	PMID:21712547
	present during mitotic M phase	experimental evidence	S476	PMID:21712547
	present during mitotic M phase	experimental evidence	S56	PMID:21712547
	present during mitotic M phase	experimental evidence	S1188	PMID:21712547
		IDA	S37	PMID:25720772
		IDA	S29	PMID:25720772
		IDA	S486	PMID:25720772
		IDA	S36	PMID:25720772
		experimental evidence	S475	PMID:24763107
		IDA	S30	PMID:25720772
		experimental evidence	S476	PMID:24763107
		experimental evidence	S486	PMID:24763107
		IDA	S38	PMID:25720772
		IDA	S27	PMID:25720772
		experimental evidence	S56	PMID:24763107
		IDA	S1188	PMID:25720772
MOD:00047	O-phospho-L-threonine				1087
	present during mitotic M phase	experimental evidence	T58	PMID:21712547
	present during mitotic M phase	experimental evidence	T471	PMID:21712547
	present during mitotic M phase	experimental evidence	T472	PMID:21712547
	present during mitotic M phase	experimental evidence	T492	PMID:21712547
	present during mitotic M phase	experimental evidence	T1186	PMID:21712547
	level fluctuates during mitotic cell cycle	experimental evidence	T472	PMID:24763107
MOD:00696	phosphorylated residue				2515
MOD:00696		IDA		PMID:19547744	2515
MOD:01149	sumoylated lysine				174
MOD:01149		IDA		PMID:26404184	174
MOD:01455	O-phosphorylated residue				2461
		IDA	S29,S30	PMID:25720772
		IDA	S30,S38	PMID:25720772
		IDA	S486,T491	PMID:25720772
		IDA	S27,S30	PMID:25720772
		IDA	S486,T492	PMID:25720772
		IDA	S36,S38	PMID:25720772
		IDA	S29,S38	PMID:25720772
		IDA	S27,S29	PMID:25720772
		IDA	S27,S36	PMID:25720772
		IDA	S37,S38	PMID:25720772
		IDA	S27,S29,S36	PMID:25720772
		IDA	S475,S476,S486	PMID:25720772
		IDA	S29,S36,S37	PMID:25720772
		IDA	S476,S486,S489	PMID:25720772
		IDA	S36,S37	PMID:25720772
		IDA	S486,S489	PMID:25720772