PomBase - Phenotype ontology term - FYPO:0000412 - abnormal cell fusion during mating - The Schizosaccharomyces pombe genome database

Annotation

Multi-locus phenotype

FYPO:0000413 - abolished cell fusion during mating

References:

PMID:25825517
PMID:31495586
PMID:35673994
PMID:41855172

Genes:

acp2 (SPAC631.01c)
cdc42 (SPAC110.03)
fus1 (SPAC20G4.02c)
leu1 (SPBC1A4.02c)
myo51 (SPBC2D10.14c)
myo52 (SPCC1919.10c)
shk2 (SPAC1F5.09c)

Genotypes:

acp2delta fus1-G1087R,N1088P(aa)
acp2delta fus1-I951A(aa)
acp2delta fus1-K1112A(aa)
acp2delta fus1-K879A(aa)
cdc42-mCherry-SW(cdc42(1-134)-linker-mCherry-linker-cdc42(135-192)) shk2delta
fus1+(wild type)[Knockdown] leu1-32(G46E aa)
fus1+(wild type)[Overexpression] leu1-32(G46E aa)
fus1N-cdc12C(fus1delta::fus1(1-792)-BamHIsite-cdc12(888-1841)) leu1-32(G46E aa)
fus1N-for3C(fus1delta::fus1(1-792)-BamHIsite-for3(715-1461)) leu1-32(G46E aa)
myo51delta myo52delta

FYPO:0007094 - increased duration of cell fusion during mating

References:

PMID:31495586

Genes:

acp1 (SPAC12B10.07)
acp2 (SPAC631.01c)

Genotypes:

acp1delta acp2delta

Single locus phenotype

FYPO:0000412 - abnormal cell fusion during mating

References:

PMID:41855172

Genes:

cdc42 (SPAC110.03)

Genotypes:

cdc42-mCherry-SW(cdc42(1-134)-linker-mCherry-linker-cdc42(135-192))
cdc42-sfGFP-SW(cdc42(1-134)-linker-sfGFP-linker-cdc42(135-192))

FYPO:0000413 - abolished cell fusion during mating

References:

PMID:11952834
PMID:19627505
PMID:24514900
PMID:28410370
PMID:30089908
PMID:31495586
PMID:34382996
PMID:35673994
PMID:36202103
PMID:39705284
PMID:41855172

Genes:

cdc42 (SPAC110.03)
cdc8 (SPAC27F1.02c)
dni1 (SPAC31G5.07)
fus1 (SPAC20G4.02c)
gpd1 (SPBC215.05)
prm1 (SPAP7G5.03)
rng8 (SPAC4H3.14c)
rng9 (SPBP8B7.02)
shk2 (SPAC1F5.09c)
slm1 (SPAC637.13c)
twf1 (SPCC126.06)

Genotypes:

cdc12N-Fus1C(Cdc12N(1-887)-BamHIsite-Fus1C(793-1372))
cdc8+(wild type)[Knockdown]
cdc8-E104A(aa)
cdc8-R121A(aa)
dni1delta
for3N-fus1C(for3N(1-714)-BamHIsite-fus1C(793-1372))
fus1(1-491)-CRY2(PHR)-fus1(792-1372)(Fus1 chimera with the IDR replaced by the light-sensitive domain of A. thaliana CRYPTOCHROME 2)
fus1(1-491)-CRY2(olig)-fus1(792-1372)(Fus1 chimera with the IDR replaced by an oligomerization-promoting mutant of the light-sensitive domain of A. thaliana CRYPTOCHROME 2)
fus1(Nt)-cdc12(1/2FH1)-cdc12(FH2)-fus1(Ct)(fus1delta::fus1(1-791)-cdc12(928-1390)-fus1(1278-1372))
fus1(Nt)-cdc12(2FH1)-cdc12(FH2)-fus1(Ct)(fus1delta::fus1(1-791)-cdc12(882-972)-cdc12(882-1390)-fus1(1278-1372))
fus1(Nt)-cdc12(2FH1)-cdc12(FH2,E1168R)-fus1(Ct)(fus1delta::fus1(1-791)-cdc12(882-972)-cdc12(882-1390,E1168R)-fus1(1278-1372),with cdc12_E1168R mutation)
fus1(Nt)-cdc12(2FH1)-fus1(FH2,R1054E)-fus1(Ct)(fus1delta::fus1(1-791)-cdc12(882-972)-cdc12(882-972)-fus1(869-1372),with fus1_R1054E mutation)
fus1(Nt)-cdc12(FH1)-cdc12(FH2)-fus1(Ct)(fus1delta::fus1(1-791)-cdc12(882-1390)-fus1(1278-1372))
fus1(Nt)-for3(FH1)-for3(FH2)-fus1(Ct)(fus1delta::fus1(1-791)-for3(718-1265)-fus1(1278-1372))
fus1(Nt)-fus1(FH1)-cdc12(FH2)-fus1(Ct)(fus1delta::fus1(1-868)-cdc12(973-1390)-fus1(1278-1372))
fus1-G1087R,N1088P(aa)
fus1-I951A(aa)
fus1-K1112A(aa)
fus1-K879A(aa)
fus1-opto(Fus1N(1-793)-A. thaliana CIBN::A. thaliana CRY2-Fus1C(794-1392))
fus1N-cdc12C(fus1delta::fus1(1-792)-BamHIsite-cdc12(888-1841))
fus1N-for3C(fus1delta::fus1(1-792)-BamHIsite-for3(715-1461))
fus1delta
fus1delta(492-749)(aa)
fus1delta(501-791)(aa)
fus1deltaIDR(492-791 Δaa)
gpd1delta
pfus1:fus1N-cdc12deltaolig(fus1N(1-792)-BamHIsite-cdc12CΔolig(888-1451),expressed from the fus1 promotor at the ura4 locus)
ppom1+prga3-cdc42(ppom1-cdc42(1-192) + ppom1-rga3(1-192))[Knockdown]
ppom1-cdc42(ppom1-cdc42(1-192))[Knockdown]
prga3-cdc42(prga3-cdc42(1-192))[Knockdown]
prm1delta
pypt1-cdc42(pypt1-cdc42(1-192))[Knockdown]
rng8delta
rng9delta
shk2delta
slm1delta
twf1delta

FYPO:0010061 - decreased cell fusion during mating

References:

PMID:27798845

Genes:

map3 (SPAC3F10.10c)
rgs1 (SPAC22F3.12c)

Genotypes:

map3-dn9(311-365 Δaa)
rgs1delta

FYPO:0007094 - increased duration of cell fusion during mating

References:

PMID:31495586
PMID:35673994
PMID:36202103

Genes:

acp1 (SPAC12B10.07)
acp2 (SPAC631.01c)
fus1 (SPAC20G4.02c)
leu1 (SPBC1A4.02c)

Genotypes:

acp1delta
acp2-R12A,Y77A(aa)
acp2delta
fus1(1-491)-FUS(G156E)-fus1(792-1372)(Fus1 chimera with the IDR replaced by the human Fused-in-Sarcoma low complexity region with the G156E mutation)
fus1(Nt)-cdc12(1/2FH1)-fus1(FH2)-fus1(Ct)(fus1delta::fus1(1-791)-cdc12(928-972)-fus1(869-1372))
fus1(Nt)-cdc12(2FH1)-fus1(FH2)-fus1(Ct)(fus1delta::fus1(1-791)-cdc12(882-972)-cdc12(882-972)-fus1(869-1372))
fus1(Nt)-fus1(2FH1)-fus1(FH2)-fus1(Ct)(fus1delta::fus1(1-868)-fus1(792-1372))
leu1-32(G46E aa)

FYPO:0006671 - transient cell fusion during mating

References:

PMID:41855172

Genes:

cdc42 (SPAC110.03)

Genotypes:

cdc42-mCherry-SW(cdc42(1-134)-linker-mCherry-linker-cdc42(135-192))

Phenotype ontology term - FYPO:0000412 - abnormal cell fusion during mating

Term summary

Parents

Annotation

Multi-locus phenotype

FYPO:0000413 - abolished cell fusion during mating

FYPO:0007094 - increased duration of cell fusion during mating

Single locus phenotype

FYPO:0000412 - abnormal cell fusion during mating

FYPO:0000413 - abolished cell fusion during mating

FYPO:0010061 - decreased cell fusion during mating

FYPO:0007094 - increased duration of cell fusion during mating

FYPO:0006671 - transient cell fusion during mating